Data/hora: 02/06/2024 06:48:13

Provedor de dados: | 56
País: | Brazil
Título: | Precipitated immune complexes of IgM induce the generation of reactive oxygen species by rabbit polymorphonuclear leucocytes
Autores: | Lucisano,Y.M.; de-Mello,A.M.; Vasques,E.R.; Mantovani,B.
Data: | 1998-06-01
Ano: | 1998
Palavras-chave: | Reactive oxygen species; Polymorphonuclear leucocytes; Immune complexes; IgM; IgG.
Resumo: | We report that immune complexes of IgM (ICIgM) antibodies and ovalbumin in the form of a precipitate from the equivalence zone induce the generation of reactive oxygen species by rabbit blood polymorphonuclear leucocytes (PMN), as measured by the chemiluminescence (CL) production in the presence of luminol. The kinetics of CL generation induced by ICIgM is quite different from that induced by precipitated immune complexes of IgG (ICIgG): the maximum rate of CL production for ICIgM occurs around 14 min, whereas for ICIgG it occurs about 5 min after incubation with the cells. Also the triggering of the process requires a higher concentration of ICIgM than of ICIgG. Evidence is presented that these effects are not mediated by interaction of the antigen (ovalbumin) with the cell, since immune precipitates of ovalbumin and the F(ab')2 fragment had no effect. Our observations that precipitated ICIgM can also be an effective stimulus for CL generation and thus for O2- production reveal a new functional capability of PMN. These results may have implications for the understanding of the participation of ICIgM (as well as of ICIgG) in inflammatory reactions mediated by PMN in immune complex diseases, and in the mechanisms of defense against microbes and other non-self agents.
Tipo: | Info:eu-repo/semantics/report
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600011
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1998000600011
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.31 n.6 1998
Fonte: | info:eu-repo/semantics/openAccess