Data/hora: 17/06/2024 14:44:34

Provedor de dados: | 56
País: | Brazil
Título: | Serotonin regulates osteoblast proliferation and function in vitro
Autores: | Dai,S.Q.; Yu,L.P.; Shi,X.; Wu,H.; Shao,P.; Yin,G.Y.; Wei,Y.Z.
Data: | 2014-09-01
Ano: | 2014
Palavras-chave: | Serotonin; Osteoblast; Proliferation; Differentiation; 5-HT receptors; 5-HT.
Resumo: | The monoamine serotonin (5-hydroxytryptamine, 5-HT), a well-known neurotransmitter, also has important functions outside the central nervous system. The objective of this study was to investigate the role of 5-HT in the proliferation, differentiation, and function of osteoblasts in vitro. We treated rat primary calvarial osteoblasts with various concentrations of 5-HT (1 nM to 10 µM) and assessed the rate of osteoblast proliferation, expression levels of osteoblast-specific proteins and genes, and the ability to form mineralized nodules. Next, we detected which 5-HT receptor subtypes were expressed in rat osteoblasts at different stages of osteoblast differentiation. We found that 5-HT could inhibit osteoblast proliferation, differentiation, and mineralization at low concentrations, but this inhibitory effect was mitigated at relatively high concentrations. Six of the 5-HT receptor subtypes (5-HT1A, 5-HT1B, 5-HT1D, 5-HT2A, 5-HT2B, and 5-HT2C) were found to exist in rat osteoblasts. Of these, 5-HT2A and 5-HT1Breceptors had the highest expression levels, at both early and late stages of differentiation. Our results indicated that 5-HT can regulate osteoblast proliferation and function in vitro.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900759
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431X20143565
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.47 n.9 2014
Fonte: | info:eu-repo/semantics/openAccess