Data/hora: 04/06/2024 05:34:03

Provedor de dados: | Brazilian Journal of Medical and Biological Research
País: | Brazil
Título: | Production and characterization of a monoclonal antibody against an Ascaris suum allergenic component
Autores: | Pires,R.R.; Oshiro,T.M.; Itami,D.M.; Fernandes,I.; Macedo-Soares,M.F.
Data: | 2001-08-01
Ano: | 2001
Palavras-chave: | Ascaris suum; Allergen; IgE; Monoclonal antibodies.
Resumo: | Ascaris suum allergenic components (PIII) separated by gel filtration chromatography of an adult worm extract were used to immunize BALB/c mice. Popliteal lymph node cells taken from the immunized animals were fused with SP2/O myeloma cells using polyethylene glycol (MW 1450) as fusogen. The hybridomas were cultured in HAT-containing medium and cloned at limiting dilutions. Supernatants from the growing hybrids were screened by ELISA using plates coated with PIII or the A. suum crude extract. The monoclonal antibody obtained, named MAC-3 (mouse anti-A. suum allergenic component), is an IgG1 kappa mouse immunoglobulin that specifically recognizes a 29,000 molecular weight protein (called allergenic protein) with an affinity constant of 1.7 x 10(9) M-1. The A. suum components recognized by MAC-3 induce specific IgE antibody production in immunized BALB/c mice. Ascitic fluid induced in Swiss mice by injecting ip the hybridoma cells and incomplete Freund's adjuvant was purified by affinity chromatography using a protein A-Sepharose column. The purified monoclonal antibody was then coupled to activated Sepharose beads in order to isolate the A. suum allergenic component from the whole extract by affinity chromatography.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800009
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2001000800009
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.34 n.8 2001
Fonte: | info:eu-repo/semantics/openAccess