Data/hora: 11/06/2024 11:03:56

Provedor de dados: | Brazilian Journal of Medical and Biological Research
País: | Brazil
Título: | Cholesterol changes the fatty acid composition of rat enterocytes
Autores: | Gazzola,J.; Martins,E.F.; Miyasaka,C.K.; Palanch,A.C.; Vecchia,M.G.; Curi,R.
Data: | 2003-01-01
Ano: | 2003
Palavras-chave: | Enterocytes; Cholesterol; Fatty acid composition; Cell proliferation.
Resumo: | The effect of free cholesterol on the fatty acid composition and growth of rat fetal enterocytes was investigated in the absence and presence of 10% (v/v) fetal calf serum. Cholesterol caused a significant reduction of cell number after 6 and 12 h in culture. The fatty acid composition of enterocytes cultured in the presence of serum was also changed by the presence of 20 µM cholesterol. The fatty acid profile was determined by HPLC using fluorescence detection (325 nm excitation and 395 nm emission). Cholesterol (20 µM) increased the proportion (given in percentage of the total fatty acids) of the following fatty acids in cultured cells: lauric (by 42%), oleic (by 34%), linoleic (by 44%) and gamma-linolenic (by 20%) acids and reduced the proportion of palmitic (by 12%), stearic (by 20%), arachidonic (by 21%) and docosahexaenoic (by 44%) acids. In addition to modifying the content of individual fatty acids, cholesterol increased the polyunsaturated/saturated fatty acid ratio from 0.48 to 0.67 and the unsaturation index from 67.12 to 75.30. This is the first evidence that cholesterol modifies fatty acid composition possibly via de novo fatty acid synthesis and desaturation.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100019
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2003000100019
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.36 n.1 2003
Fonte: | info:eu-repo/semantics/openAccess