Data/hora: 16/06/2024 07:10:41

Provedor de dados: 	56	
País: 	Brazil	
Título: 	Up-regulated expression of CD147 gene in malignant bone tumor and the possible induction mechanism during osteoclast formation	
Autores: 	Qiao,Suchi; Liu,Chang; Xu,Weijie; AZhaTi,WuBuLi; Li,Cheng; Wang,Zhiwei.	
Data: 	2018-01-01	
Ano: 	2018	
Palavras-chave: 	Bone cancer; RT-PCR; Western blot; RANKL; Human.	
Resumo: 	It is increasingly evident that the microenvironment of bone can influence cancer phenotype in many ways that favor growth in bone. CD147, a transmembrane protein of the immunoglobulin (Ig) superfamily, was identified independently in different species and has many designations across different species. However, expression levels of CD147 mRNA in bone cancer have not been described. In this study, we have used real-time fluorescence quantification (RT-PCR) to demonstrate CD147 expression in malignant bone cancer and benign bone tumor tissues. The results suggested that the expression of CD147 gene was significantly up-regulated in malignant bone cancer. Moreover, we found that over-expressed RANKL progressively enhanced osteoclast formation up to 48 h, which suggested that RANKL could promote the formation of osteoclast, indicating that both CD147 and RANKL play important roles in the formation of osteoclasts. Furthermore, the expressions of four osteoclast specific expression genes, including TRACP, MMP-2, MMP-9 and c-Src, were analyzed using RT-PCR. The results indicated that four osteoclast-specific expression genes were detectable in all osteoclast with different treatments. However, the highest expression level of these four osteoclast-specific expression genes appears in the CD147+ RANKL group and the lowest expression level of these four osteoclast-specific expression genes appears with si-RANKL treatment. Characterization of the role of CD147 in the development of tumors should lead to a better understanding of the changes occurring at the molecular level during the development and progression of primary human bone cancer.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900608	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/1414-431x20186948	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.51 n.9 2018	
Fonte: 	info:eu-repo/semantics/openAccess