Data/hora: 09/06/2024 09:23:23

Provedor de dados: | Brazilian Journal of Medical and Biological Research
País: | Brazil
Título: | The effect of bacterial lipopolysaccharide on gastric emptying in rats suffering from moderate renal insufficiency
Autores: | Rigatto,S.Z.P.; Collares,E.F.
Data: | 1998-04-01
Ano: | 1998
Palavras-chave: | Gastric emptying; Lipopolysaccharide; Endotoxin; Renal insufficiency; Rats.
Resumo: | The objective of the present study was to evaluate the response of rats suffering from moderate renal insufficiency to bacterial lipopolysaccharide (LPS, or endotoxin). The study involved 48 eight-week-old male SPF Wistar rats (175-220 g) divided into two groups of 24 animals each. One group underwent 5/6 nephrectomy while the other was sham-operated. Two weeks after surgery, the animals were further divided into two subgroups of 12 animals each and were fasted for 20 h but with access to water ad libitum. One nephrectomized and one sham-treated subgroup received E. coli LPS (25 µg/kg, iv) while the other received a sterile, pyrogen-free saline solution. Gastric retention (GR) was determined 10 min after the orogastric infusion of a standard saline test meal labeled with phenol red (6 mg/dl). The gastric emptying of the saline test meal was studied after 2 h. Renal function was evaluated by measuring the plasma levels of urea and creatinine. The levels of urea and creatinine in 5/6 nephrectomized animals were two-fold higher than those observed in the sham-operated rats. Although renal insufficiency did not change gastric emptying (median %GR = 26.6 for the nephrectomized subgroup and 29.3 for the sham subgroup), LPS significantly retarded the gastric emptying of the sham and nephretomized groups (median %GR = 42.0 and 61.0, respectively), and was significantly greater (P&amp;lt;0.01) in the nephrectomized rats. We conclude that gastric emptying in animals suffering from moderate renal insufficiency is more sensitive to the action of LPS than in sham animals
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400006
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1998000400006
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.31 n.4 1998
Fonte: | info:eu-repo/semantics/openAccess