Data/hora: 11/06/2024 05:34:21

Provedor de dados: | Brazilian Journal of Medical and Biological Research
País: | Brazil
Título: | Interaction between growth differentiation factor 9, insulin-like growth factor I and growth hormone on the in vitro development and survival of goat preantral follicles
Autores: | Martins,F.S.; Celestino,J.J.H.; Saraiva,M.V.A.; Chaves,R.N.; Rossetto,R.; Silva,C.M.G.; Lima-Verde,I.B.; Lopes,C.A.P.; Campello,C.C.; Figueiredo,J.R.
Data: | 2010-08-01
Ano: | 2010
Palavras-chave: | GDF-9; IGF-I; GH; Culture; Follicle activation; Goat.
Resumo: | The objective of this study was to determine the effects of GDF-9, IGF-I, and GH alone or combined on preantral follicle survival, activation and development after 1 and 7 days of in vitro culture. Either fresh (non-cultured) or cultured ovarian tissue was processed for histological and fluorescence analysis. For all media tested, the percent of normal follicles was greater when compared to minimum essential medium supplemented (MEM+) alone, except when ovarian tissue was cultured with GDF-9/IGF-I or GDF-9/GH (P &lt; 0.05). Fluorescence analysis showed that the percent of viable follicles after 7 days of culture was similar for non-cultured tissue and for all treatments tested. The percent of primordial follicles was reduced (P &lt; 0.05) and there was a significant and concomitant increase in the percent of intermediate and primary follicles in all treatments tested after 7 days of culture when compared to non-cultured tissue. After 7 days of culture, the highest percent of intermediate follicles was observed with IGF-I/GH (61.3%), and the highest percent of primary follicles was achieved with IGF-I (57.7%). After 7 days of culture in MEM+ containing GDF-9, IGF-I and GH alone or in all associations, a significant increase in follicular diameter was observed when compared to MEM+ alone and non-cultured tissue. In conclusion, GDF-9, IGF-I and GH alone or in combination maintain preantral follicle survival and promote primordial follicle activation. Nevertheless, the data showed that IGF-I/GH and IGF-I alone are efficient in promoting the transition from primordial to intermediate follicles and from intermediate to primary follicles, respectively.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800006
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2010007500066
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.43 n.8 2010
Fonte: | info:eu-repo/semantics/openAccess