Data/hora: 10/06/2024 10:20:22

Provedor de dados: | 56
País: | Brazil
Título: | Early neonatal echocardiographic findings in an experimental rabbit model of congenital diaphragmatic hernia
Autores: | Manso,P.H.; Figueira,R.L.; Prado,C.M.; Gonçalves,F.L.; Simões,A.L.B.; Ramos,S.G.; Sbragia,L.
Data: | 2015-03-01
Ano: | 2015
Palavras-chave: | Congenital diaphragmatic hernia; Right ventricle; Pulmonary arterial hypertension; Echocardiography.
Resumo: | This study aimed to demonstrate that congenital diaphragmatic hernia (CDH) results in vascular abnormalities that are directly associated with the severity of pulmonary hypoplasia and hypertension. These events increase right ventricle (RV) afterload and may adversely affect disease management and patient survival. Our objective was to investigate cardiac function, specifically right ventricular changes, immediately after birth and relate them to myocardial histological findings in a CDH model. Pregnant New Zealand rabbits underwent the surgical procedure at 25 days of gestation (n=14). CDH was created in one fetus per horn (n=16), and the other fetuses were used as controls (n=20). At term (30 days), fetuses were removed, immediately dried and weighed before undergoing four-parameter echocardiography. The lungs and the heart were removed, weighed, and histologically analyzed. CDH animals had smaller total lung weight (P&lt;0.005), left lung weight (P&lt;0.005), and lung-to-body ratio (P&lt;0.005). Echocardiography revealed a smaller left-to-right ventricle ratio (LV/RV, P&lt;0.005) and larger diastolic right ventricle size (DRVS, P&lt;0.007). Histologic analysis revealed a larger number of myocytes undergoing mitotic division (186 vs 132, P&lt;0.05) in CDH hearts. Immediate RV dilation of CDH hearts is related to myocyte mitosis increase. This information may aid the design of future strategies to address pulmonary hypertension in CDH.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300234
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431x20144184
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.48 n.3 2015
Fonte: | info:eu-repo/semantics/openAccess