Data/hora: 10/06/2024 18:33:01

Provedor de dados: | 56
País: | Brazil
Título: | Reversal by methylene blue of tetanic fade induced in cats by nitric oxide
Autores: | Ambiel,C.R.; Alves-Do-Prado,W.
Data: | 1998-03-01
Ano: | 1998
Palavras-chave: | Wedensky inhibition; Nitric oxide; Methylene blue; Skeletal muscle.
Resumo: | Previous data from our laboratory have indicated that nitric oxide (NO) acting at the presynaptic level increases the amplitude of muscular contraction (AMC) of the phrenic-diaphragm preparations isolated from indirectly stimulated rats, but, by acting at the postsynaptic level, it reduces the AMC when the preparations are directly stimulated. In the present study we investigated the effects induced by NO when tetanic frequencies of stimulation were applied to in vivo preparations (sciatic nerve-anterior tibial muscle of the cat). Intra-arterial injection of NO (0.75-1.5 mg/kg) induced a dose-dependent increase in the Wedensky inhibition produced by high frequencies of stimulation applied to the motor nerve. Intra-arterial administration of 7.2 µg/kg methylene blue did not produce any change in AMC at low frequencies of nerve stimulation (0.2 Hz), but antagonized the NO-induced Wedensky inhibition. The experimental data suggest that NO-induced Wedensky inhibition may be mediated by the guanylate cyclase-cGMP pathway
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300013
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1998000300013
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.31 n.3 1998
Fonte: | info:eu-repo/semantics/openAccess