Data/hora: 09/06/2024 06:00:11

Provedor de dados: | 56
País: | Brazil
Título: | Effect of hepatocyte growth factor and angiotensin II on rat cardiomyocyte hypertrophy
Autores: | Chen,Ai-Lan; Ou,Cai-Wen; He,Zhao-Chu; Liu,Qi-Cai; Dong,Qi; Chen,Min-Sheng.
Data: | 2012-12-01
Ano: | 2012
Palavras-chave: | Angiotensin II; Hepatocyte growth factor; Cardiomyocyte; Hypertrophy.
Resumo: | Angiotensin II (Ang II) plays an important role in cardiomyocyte hypertrophy. The combined effect of hepatocyte growth factor (HGF) and Ang II on cardiomyocytes is unknown. The present study was designed to determine the effect of HGF on cardiomyocyte hypertrophy and to explore the combined effect of HGF and Ang II on cardiomyocyte hypertrophy. Primary cardiomyocytes were isolated from neonatal rat hearts and cultured in vitro. Cells were treated with Ang II (1 µM) alone, HGF (10 ng/mL) alone, and Ang II (1 µM) plus HGF (10 ng/mL) for 24, 48, and 72 h. The amount of [³H]-leucine incorporation was then measured to evaluate protein synthesis. The mRNA levels of β-myosin heavy chain and atrial natriuretic factor were determined by real-time PCR to evaluate the presence of fetal phenotypes of gene expression. The cell size of cardiomyocytes was also studied. Ang II (1 µM) increased cardiomyocyte hypertrophy. Similar to Ang II, treatment with 1 µM HGF promoted cardiomyocyte hypertrophy. Moreover, the combination of 1 µM Ang II and 10 ng/mL HGF clearly induced a combined pro-hypertrophy effect on cardiomyocytes. The present study demonstrates for the first time a novel, combined effect of HGF and Ang II in promoting cardiomyocyte hypertrophy.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200007
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2012007500159
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.45 n.12 2012
Fonte: | info:eu-repo/semantics/openAccess