Data/hora: 02/06/2024 09:40:42

Provedor de dados: 	56	
País: 	Brazil	
Título: 	Nitric oxide modulates ATP-evoked currents in mouse Leydig cells	
Autores: 	de Deus,J.L.; Dagostin,A.L.A.; Varanda,W.A.	
Data: 	2018-01-01	
Ano: 	2018	
Palavras-chave: 	Leydig cells; ATP; Purinergic receptor; Calcium; Nitric oxide; Whole cell patch clamp.	
Resumo: 	Testosterone synthesis within Leydig cells is a calcium-dependent process. Intracellular calcium levels are regulated by different processes including ATP-activated P2X purinergic receptors, T-type Ca2+ channels modulated by the luteinizing hormone, and intracellular calcium storages recruited by a calcium-induced calcium release mechanism. On the other hand, nitric oxide (NO) is reported to have an inhibitory role in testosterone production. Based on these observations, we investigated the interaction between the purinergic and nitrergic systems in Leydig cells of adult mice. For this purpose, we recorded ATP-evoked currents in isolated Leydig cells using the whole cell patch clamp technique after treatment with L-NAME (300 μM and 1 mM), L-arginine (10, 100, 300, and 500 μM), ODQ (300 μM), and 8-Br-cGMP (100 μM). Our results show that NO produced by Leydig cells in basal conditions is insufficient to change the ATP-evoked currents and that extra NO provided by adding 300 μM L-arginine positively modulates the current through a mechanism involving the NO/cGMP signaling pathway. Thus, we report an interaction between the nitrergic and purinergic systems in Leydig cells and suggest that Ca2+ entry via the purinergic receptors can be regulated by NO.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500603	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/1414-431x20186693	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.51 n.5 2018	
Fonte: 	info:eu-repo/semantics/openAccess