Data/hora: 11/06/2024 12:28:17

Provedor de dados: | Brazilian Journal of Medical and Biological Research
País: | Brazil
Título: | Lipid peroxidation and total radical-trapping potential of the lungs of rats submitted to chronic and sub-chronic stress
Autores: | Torres,R.L.; Torres,I.L.S.; Gamaro,G.D.; Fontella,F.U.; Silveira,P.P.; Moreira,J.S.R.; Lacerda,M.; Amoretti,J.R.; Rech,D.; Dalmaz,C.; Belló,A.A.
Data: | 2004-02-01
Ano: | 2004
Palavras-chave: | Stress; TBARS; TRAP; Free radicals; Lungs; Oxidative stress.
Resumo: | Exposure to stress induces a cluster of physiological and behavioral changes in an effort to maintain the homeostasis of the organism. Long-term exposure to stress, however, has detrimental effects on several cell functions such as the impairment of antioxidant defenses leading to oxidative damage. Oxidative stress is a central feature of many diseases. The lungs are particularly susceptible to lesions by free radicals and pulmonary antioxidant defenses are extensively distributed and include both enzymatic and non-enzymatic systems. The aim of the present study was to determine lipid peroxidation and total radical-trapping potential (TRAP) changes in lungs of rats submitted to different models of chronic stress. Adult male Wistar rats weighing 180-230 g were submitted to different stressors (variable stress, N = 7) or repeated restraint stress for 15 (N = 10) or 40 days (N = 6) and compared to control groups (N = 10 each). Lipid peroxidation levels were assessed by thiobarbituric acid reactive substances (TBARS), and TRAP was measured by the decrease in luminescence using the 2-2'-azo-bis(2-amidinopropane)-luminol system. Chronic variable stress induced a 51% increase in oxidative stress in lungs (control group: 0.037 ± 0.002; variable stress: 0.056 ± 0.007, P &lt; 0.01). No difference in TBARS was observed after chronic restraint stress, but a significant 57% increase in TRAP was presented by the group repeatedly restrained for 15 days (control group: 2.48 ± 0.42; stressed: 3.65 ± 0.16, P &lt; 0.05). We conclude that different stressors induce different effects on the oxidative status of the organism.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200004
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2004000200004
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.37 n.2 2004
Fonte: | info:eu-repo/semantics/openAccess