Data/hora: 08/06/2024 06:39:42

Provedor de dados: | 56
País: | Brazil
Título: | Thalidomide protects mice against LPS-induced shock
Autores: | Moreira,A.L.; Wang,J.; Sarno,E.N.; Kaplan,G.
Data: | 1997-10-01
Ano: | 1997
Palavras-chave: | Endotoxic shock; Thalidomide; Cytokines; Inflammation.
Resumo: | Thalidomide has been shown to selectively inhibit TNF-&lt;!-- $MVD$:face("Symbol") --&gt;&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt; production in vitro by lipopolysaccharide (LPS)-stimulated monocytes. TNF-&lt;!-- $MVD$:face("Symbol") --&gt;&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt; has been shown to play a pivotal role in the pathophysiology of endotoxic shock. Using a mouse model of LPS-induced shock, we investigated the effects of thalidomide on the production of TNF-&lt;!-- $MVD$:face("Symbol") --&gt;&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt; and other cytokines and on animal survival. After injection of 100-350 µg LPS into mice, cytokines including TNF-&lt;!-- $MVD$:face("Symbol") --&gt;&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;, IL-6, IL-10, IL-1ß, GM-CSF and IFN-&lt;!-- $MVD$:face("Symbol") --&gt;&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; were measured in the serum. Administration of 200 mg/kg thalidomide to mice before LPS challenge modified the profile of LPS-induced cytokine secretion. Serum TNF-&lt;!-- $MVD$:face("Symbol") --&gt;&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt; levels were reduced by 93%, in a dose-dependent manner, and TNF-&lt;!-- $MVD$:face("Symbol") --&gt;&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt; mRNA expression in the spleens of mice was reduced by 70%. Serum IL-6 levels were also inhibited by 50%. Thalidomide induced a two-fold increase in serum IL-10 levels. Thalidomide treatment did not interfere with the production of GM-CSF, IL-1ß or IFN-&lt;!-- $MVD$:face("Symbol") --&gt;&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt;. The LD50 of LPS in this model was increased by thalidomide pre-treatment from 150 µg to 300 µg in 72 h. Thus, at otherwise lethal doses of LPS, thalidomide treatment was found to protect animals from death
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000010
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1997001000010
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.30 n.10 1997
Fonte: | info:eu-repo/semantics/openAccess