Data/hora: 10/06/2024 09:18:42

Provedor de dados: | Brazilian Journal of Medical and Biological Research
País: | Brazil
Título: | An electronic pressure-meter nociception paw test for mice
Autores: | Cunha,T.M.; Verri Jr.,W.A.; Vivancos,G.G.; Moreira,I.F.; Reis,S.; Parada,C.A.; Cunha,F.Q.; Ferreira,S.H.
Data: | 2004-03-01
Ano: | 2004
Palavras-chave: | Hyperalgesia; Nociception; Indomethacin; Dipyrone; Von Frey filaments.
Resumo: | The aim of the present investigation was to describe and validate an electronic mechanical test for quantification of the intensity of inflammatory nociception in mice. The electronic pressure-meter test consists of inducing the animal hindpaw flexion reflex by poking the plantar region with a polypropylene pipette tip adapted to a hand-held force transducer. This method was compared to the classical von Frey filaments test in which pressure intensity is automatically recorded after the nociceptive hindpaw flexion reflex. The electronic pressure-meter and the von Frey filaments were used to detect time versus treatment interactions of carrageenin-induced hypernociception. In two separate experiments, the electronic pressure-meter was more sensitive than the von Frey filaments for the detection of the increase in nociception (hypernociception) induced by small doses of carrageenin (30 µg). The electronic pressure-meter detected the antinociceptive effect of non-steroidal drugs in a dose-dependent manner. Indomethacin administered intraperitoneally (1.8-15 mg/kg) or intraplantarly (30-300 µg/paw) prevented the hypersensitive effect of carrageenin (100 µg/paw). The electronic pressure-meter also detected the hypernociceptive effect of prostaglandin E2 (PGE2; 10-100 ng) in a dose-dependent manner. The hypernociceptive effect of PGE2 (100 ng) was blocked by dipyrone (160 and 320 µg/paw) but not by intraplantar administration of indomethacin (300 µg/paw). The present results validate the use of the electronic pressure-meter as more sensitive than the von Frey filaments in mice. Furthermore, it is an objective and quantitative nociceptive test for the evaluation of the peripheral antinociceptive effect of anti-inflammatory analgesic drugs, which inhibit prostaglandin synthesis (indomethacin) or directly block the ongoing hypernociception (dipyrone).
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300018
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2004000300018
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.37 n.3 2004
Fonte: | info:eu-repo/semantics/openAccess