Data/hora: 18/06/2024 18:12:34

Provedor de dados: | Brazilian Journal of Medical and Biological Research
País: | Brazil
Título: | Observation of the effect of bone marrow mesenchymal stem cell transplantation by different interventions on cirrhotic rats
Autores: | Zhou,Xiaoling; Yang,Jianqing; Liu,Ying; Li,Zepeng; Yu,Jingfang; Wei,Wanhua; Chen,Qiao; Li,Can; Tang,Nong.
Data: | 2019-01-01
Ano: | 2019
Palavras-chave: | Bone marrow mesenchymal stem cells; Transplantation; Liver cirrhosis; Granulocyte colony-stimulating factor; Jisheng Shenqi decoction.
Resumo: | Bone marrow mesenchymal stem cells (BMSCs) transplantation has attracted attention for the treatment of liver cirrhosis and end-stage liver diseases. Therefore, in this study, we evaluated the effect of different methods of BMSCs transplantation in the treatment of liver cirrhosis in rats. Seventy-two male Sprague-Dawley rats were divided into 7 groups: 10 were used to extract BMSCs, 10 were used as normal group, and the remaining 52 rats were randomly divided into five groups for testing: control group, BMSCs group, BMSCs+granulocyte colony-stimulating factor (G-CSF) group, and BMSCs+Jisheng Shenqi decoction (JSSQ) group. After the end of the intervention course, liver tissue sections of rats were subjected to hematoxylin and eosin (H&amp;E) and Masson staining, and pathological grades were scored. Liver function [aminotransferase (ALT), aspartate aminotransferase (AST), albumin (ALB)] and hepatic fibrosis markers [hyaluronidase (HA), laminin (LN), type III procollagen (PCIII), type IV collagen (CIV)] were measured. BMSCs+JSSQ group had the best effect of reducing ALT and increasing ALB after intervention therapy (P&lt;0.05). The reducing pathological scores and LN, PCIII, CIV of BMSCs+G-CSF group and BMSCs+JSSQ group after intervention therapy were significant, but there was no significant difference between the two groups (P&gt;0.05). The effect of JSSQ on improving stem cell transplantation in rats with liver cirrhosis was confirmed. JSSQ combined with BMSCs could significantly improve liver function and liver pathology scores of rats with liver cirrhosis.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000300606
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431x20187879
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.52 n.3 2019
Fonte: | info:eu-repo/semantics/openAccess