Data/hora: 09/06/2024 18:03:48

Provedor de dados: | Brazilian Journal of Medical and Biological Research
País: | Brazil
Título: | Further biochemical characterization of Mycobacterium leprae laminin-binding proteins
Autores: | Marques,M.A.M.; Mahapatra,S.; Sarno,E.N.; Santos,S.; Spencer,J.S.; Brennan,P.J.; Pessolani,M.C.V.
Data: | 2001-04-01
Ano: | 2001
Palavras-chave: | Mycobacterium leprae; Laminin; Adhesion; Schwann cell; Ribosomal protein; Histone.
Resumo: | It has been demonstrated that the alpha2 chain of laminin-2 present on the surface of Schwann cells is involved in the process of attachment of Mycobacterium leprae to these cells. Searching for M. leprae laminin-binding molecules, in a previous study we isolated and characterized the cationic proteins histone-like protein (Hlp) and ribosomal proteins S4 and S5 as potential adhesins involved in M. leprae-Schwann cell interaction. Hlp was shown to bind alpha2-laminins and to greatly enhance the attachment of mycobacteria to ST88-14 Schwann cells. In the present study, we investigated the laminin-binding capacity of the ribosomal proteins S4 and S5. The genes coding for these proteins were PCR amplified and their recombinant products were shown to bind alpha2-laminins in overlay assays. However, when tested in ELISA-based assays and in adhesion assays with ST88-14 cells, in contrast to Hlp, S4 and S5 failed to bind laminin and act as adhesins. The laminin-binding property and adhesin capacity of two basic host-derived proteins were also tested, and only histones, but not cytochrome c, were able to increase bacterial attachment to ST88-14 cells. Our data suggest that the alanine/lysine-rich sequences shared by Hlp and eukaryotic H1 histones might be involved in the binding of these cationic proteins to laminin.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400004
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2001000400004
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.34 n.4 2001
Fonte: | info:eu-repo/semantics/openAccess