Data/hora: 08/06/2024 18:56:33

Provedor de dados: | Brazilian Journal of Medical and Biological Research
País: | Brazil
Título: | Immune cells and oxidative stress in the endotoxin tolerance mouse model
Autores: | Melo,E.S.; Barbeiro,H.V.; Ariga,S.; Goloubkova,T.; Curi,R.; Velasco,I.T.; Vasconcelos,D.; Soriano,F.G.
Data: | 2010-01-01
Ano: | 2010
Palavras-chave: | Sepsis; Cytokines; Immune tolerance; Lipopolysaccharide; Murine sepsis model; T helper cells.
Resumo: | Sepsis is a systemic inflammatory response that can lead to tissue damage and death. In order to increase our understanding of sepsis, experimental models are needed that produce relevant immune and inflammatory responses during a septic event. We describe a lipopolysaccharide tolerance mouse model to characterize the cellular and molecular alterations of immune cells during sepsis. The model presents a typical lipopolysaccharide tolerance pattern in which tolerance is related to decreased production and secretion of cytokines after a subsequent exposure to a lethal dose of lipopolysaccharide. The initial lipopolysaccharide exposure also altered the expression patterns of cytokines and was followed by an 8- and a 1.5-fold increase in the T helper 1 and 2 cell subpopulations. Behavioral data indicate a decrease in spontaneous activity and an increase in body temperature following exposure to lipopolysaccharide. In contrast, tolerant animals maintained production of reactive oxygen species and nitric oxide when terminally challenged by cecal ligation and puncture (CLP). Survival study after CLP showed protection in tolerant compared to naive animals. Spleen mass increased in tolerant animals followed by increases of B lymphocytes and subpopulation Th1 cells. An increase in the number of stem cells was found in spleen and bone marrow. We also showed that administration of spleen or bone marrow cells from tolerant to naive animals transfers the acquired resistance status. In conclusion, lipopolysaccharide tolerance is a natural reprogramming of the immune system that increases the number of immune cells, particularly T helper 1 cells, and does not reduce oxidative stress.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100009
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2009007500027
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.43 n.1 2010
Fonte: | info:eu-repo/semantics/openAccess