Data/hora: 09/06/2024 10:37:25

Provedor de dados: 	56	
País: 	Brazil	
Título: 	Techniques of radioautography for medical and biological research	
Autores: 	Nagata,T.	
Data: 	1998-02-01	
Ano: 	1998	
Palavras-chave: 	Macroscopic radioautography; Light microscopic radioautography; Electron microscopic radioautography; Methodology.	
Resumo: 	Standard techniques for radioautography used in biological and medical research can be classified into three categories, i.e., macroscopic radioautography, light microscopic radioautography and electron microscopic radioautography. The routine techniques used in these three procedures are described. With regard to macroscopic radioautography, whole body radioautography is a standard technique which employs freezing and cryosectioning and can demonstrate organ distributions of both soluble and insoluble compounds. In contrast, in light and electron microscopic radioautography, soluble and insoluble techniques are separated. In order to demonstrate insoluble labeled compounds, conventional chemical fixations such as formalin for light microscopy or buffered glutaraldehyde and osmium tetroxide for both light and electron microscopy followed by dehydration, embedding and wet-mounting applications of radioautographic emulsions can be used. For the demonstration of soluble labeled compounds, however, cryotechniques such as cryofixation, cryosectioning, freeze-drying, freeze-substitution followed by dry-sectioning and dry-mounting radioautography should be employed both for light and electron microscopy. The outlines of these techniques, which should be utilized in various fields of biological and medical research, are described in detail	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200002	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/S0100-879X1998000200002	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.31 n.2 1998	
Fonte: 	info:eu-repo/semantics/openAccess