Data/hora: 09/06/2024 11:23:17

Provedor de dados: | Brazilian Journal of Medical and Biological Research
País: | Brazil
Título: | Rat Dlx5 is expressed in the subventricular zone and promotes neuronal differentiation
Autores: | Shu,H.F.; Gao,F.Y.; Zhang,C.Q.; Liu,S.Y.; Zhang,Z.Y.; Song,Y.C.; Qiu,K.J.; Yang,H.
Data: | 2010-02-01
Ano: | 2010
Palavras-chave: | Neural precursors; Dlx5; Green fluorescent protein; Recombinant plasmids.
Resumo: | The molecular mechanisms and potential clinical applications of neural precursor cells have recently been the subject of intensive study. Dlx5, a homeobox transcription factor related to the distal-less gene in Drosophila, was shown to play an important role during forebrain development. The subventricular zone (SVZ) in the adult brain harbors the largest abundance of neural precursors. The anterior SVZ (SVZa) contains the most representative neural precursors in the SVZ. Further research is necessary to elucidate how Dlx5-related genes regulate the differentiation of SVZa neural precursors. Here, we employed immunohistochemistry and molecular biology techniques to study the expression of Dlx5 and related homeobox genes Er81 and Islet1 in neonatal rat brain and in in vitro cultured SVZa neural precursors. Our results show that Dlx5 and Er81 are also highly expressed in the SVZa, rostral migratory stream, and olfactory bulb. Islet1 is only expressed in the striatum. In cultured SVZa neural precursors, Dlx5 mRNA expression gradually decreased with subsequent cell passages and was completely lost by passage four. We also transfected a Dlx5 recombinant plasmid and found that Dlx5 overexpression promoted neuronal differentiation of in vitro cultured SVZa neural precursors. Taken together, our data suggest that Dlx5 plays an important role during neuronal differentiation.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200008
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2009007500034
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.43 n.2 2010
Fonte: | info:eu-repo/semantics/openAccess