Data/hora: 10/06/2024 07:01:09

Provedor de dados: | 56
País: | Brazil
Título: | Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide
Autores: | Goldstein,J.; Silberstein,C.; Ibarra,C.
Data: | 2002-02-01
Ano: | 2002
Palavras-chave: | Adenylyl cyclase; Nitric oxide; Sodium nitroprusside; Cyclic AMP; COS-7 cells; Signal transduction.
Resumo: | Adenylyl cyclase (AC) isoforms catalyze the synthesis of 3',5'-cyclic AMP from ATP. These isoforms are critically involved in the regulation of gene transcription, metabolism, and ion channel activity among others. Nitric oxide (NO) is a gaseous product whose synthesis from L-arginine is catalyzed by the enzyme NO synthase. It has been well established that NO activates the enzyme guanylyl cyclase, but little has been reported on the effects of NO on other important second messengers, such as AC. In the present study, the effects of sodium nitroprusside (SNP), a nitric oxide-releasing compound, on COS-7 cells transfected with plasmids containing AC types I, II, V and VI were evaluated. Total inhibition (~98.5%) of cAMP production was observed in COS-7 cells transfected with the AC I isoform and previously treated with SNP (10 mM) for 30 min, when stimulated with ionomycin. A high inhibition (~76%) of cAMP production was also observed in COS-7 cells transfected with the AC VI isoform and previously treated with SNP (10 mM) for 30 min, when stimulated with forskolin. No effect on cAMP production was observed in cells transfected with AC isoforms II and V.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200002
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2002000200002
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.35 n.2 2002
Fonte: | info:eu-repo/semantics/openAccess