Data/hora: 10/06/2024 15:20:15

Provedor de dados: | 56
País: | Brazil
Título: | The acute effects of strength, endurance and concurrent exercises on the Akt/mTOR/p70S6K1 and AMPK signaling pathway responses in rat skeletal muscle
Autores: | Souza,E.O.de; Tricoli,V.; Bueno Junior,C.; Pereira,M.G.; Brum,P.C.; Oliveira,E.M.; Roschel,H.; Aoki,M.S.; Urginowitsch,C.
Data: | 2013-04-01
Ano: | 2013
Palavras-chave: | Strength exercise; Concurrent training; Cell signaling; AMPK.
Resumo: | The activation of competing intracellular pathways has been proposed to explain the reduced training adaptations after concurrent strength and endurance exercises (CE). The present study investigated the acute effects of CE, strength exercises (SE), and endurance exercises (EE) on phosphorylated/total ratios of selected AMPK and Akt/mTOR/p70S6K1 pathway proteins in rats. Six animals per exercise group were killed immediately (0 h) and 2 h after each exercise mode. In addition, 6 animals in a non-exercised condition (NE) were killed on the same day and under the same conditions. The levels of AMPK, phospho-Thr172AMPK (p-AMPK), Akt, phospho-Ser473Akt (p-Akt), p70S6K1, phospho-Thr389-p70S6K1(p-p70S6K1), mTOR, phospho-Ser2448mTOR (p-mTOR), and phospho-Thr1462-TSC2 (p-TSC2) expression were evaluated by immunoblotting in total plantaris muscle extracts. The only significant difference detected was an increase (i.e., 87%) in Akt phosphorylated/total ratio in the CE group 2 h after exercise compared to the NE group (P = 0.002). There were no changes in AMPK, TSC2, mTOR, or p70S6K1 ratios when the exercise modes were compared to the NE condition (P ≥ 0.05). In conclusion, our data suggest that low-intensity and low-volume CE might not blunt the training-induced adaptations, since it did not activate competing intracellular pathways in an acute bout of strength and endurance exercises in rat skeletal muscle.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000400343
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431X20132557
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.46 n.4 2013
Fonte: | info:eu-repo/semantics/openAccess