Data/hora: 12/06/2024 10:01:26

Provedor de dados: | 56
País: | Brazil
Título: | PCR identification of Mycobacterium tuberculosis complex in a clinical sample from a patient with symptoms of tuberculous spondylodiscitis
Autores: | Ribeiro,M.A.; Barouni,A.S.; Augusto,C.J.; Augusto,M.V.S.; Lopes,M.T.P.; Salas,C.E.
Data: | 2007-01-01
Ano: | 2007
Palavras-chave: | Spondylodiscitis; Tuberculosis; Polymerase chain reaction; Mycobacterium tuberculosis complex.
Resumo: | A 42-year-old male complaining of thoracic spine pain was admitted to the hospital for evaluation. An X-ray and computer tomography of the thoracic spine showed spondylodiscitis of the L3 lumbar and L2-L3 intervertebral disk. The tuberculin skin test (PPD) was strongly positive. A radioscopy-guided fine needle aspirate of the affected area was cultured but did not reveal the cause of the disease. Two biopsy attempts failed to reveal the cause of the disease by culturing or by acid-fast-resistant staining (Ziehl Neelsen) of the specimens. A third biopsy also failed to detect the infectious agent by using microbiological procedures, but revealed the presence of a 245-bp amplicon characteristic of the Mycobacterium tuberculosis complex after PCR of the sample. The result demonstrates the efficacy of PCR for the identification of M. tuberculosis in situations in which conventional diagnosis by culturing techniques or direct microscopy is unable to detect the microorganism. Following this result the patient was treated with the antituberculous cocktail composed by rifampicin, pirazinamide and isoniazid during a six-month period. At the end of the treatment the dorsalgia symptoms had disappeared.
Tipo: | Info:eu-repo/semantics/report
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100001
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2006005000033
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.40 n.1 2007
Fonte: | info:eu-repo/semantics/openAccess