Data/hora: 29/05/2024 02:13:37

Provedor de dados: | Brazilian Journal of Medical and Biological Research
País: | Brazil
Título: | Inhibition of cytohesin-1 by siRNA leads to reduced IGFR signaling in prostate cancer
Autores: | Weizhong,Zhang; Shuohui,Gao; Hanjiao,Qin; Yuhong,Man; Xiaohua,Yang; Jian,Cao; Lisen,Li.
Data: | 2011-07-01
Ano: | 2011
Palavras-chave: | Cytohesins; Insulin-like growth factor 1 receptor; Prostate cancer; Signal transduction; SiRNA interference.
Resumo: | To explore how cytohesin-1 (CYTH-1) small interfering RNA (siRNA) influences the insulin-like growth factor receptor (IGFR)-associated signal transduction in prostate cancer, we transfected human prostate cancer PC-3 cell lines with liposome-encapsulatedCYTH-1 siRNA in serum-free medium and exposed the cells to 100 nM IGF-1. The mRNA and protein levels of the signal molecules involved in the IGFR signaling pathways were determined by real-time PCR and detected by Western blotting. The relative mRNA levels of CYTH-1, c-Myc, cyclinD1 and IGF-1R (CYTH-1 siRNA group vs scrambled siRNA group) were 0.26 vs 0.97, 0.34 vs 1.06, 0.10 vs 0.95, and 0.27 vs 0.41 (P &lt; 0.05 for all), respectively. The relative protein levels of CYTH-1, pIGF-1R, pIRS1, pAkt1, pErk1, c-Myc, and cyclinD1 (CYTH-1 siRNA group vsscrambled siRNA group) were 0.10 vs 1.00 (30 min), 0.10 vs 0.98 (30 min), 0.04 vs 0.50 (30 min), 0.10 vs 1.00 (30 min), 0.10 vs 1.00 (30 min), 0.13 vs 0.85 (5 h), and 0.08 vs 0.80 (7 h), respectively. The tyrosine kinase activity of IGF-1R was associated with CYTH-1. The proliferative activity of PC-3 cells transfected with CYTH-1 siRNA was significantly lower than that of cells transfected with scrambled siRNA at 48 h (40.5 vs87.6%, P &lt; 0.05) and at 72 h (34.5 vs 93.5%, P &lt; 0.05). In conclusion, the interference of siRNA with cytohesin-1 leads to reduced IGFR signaling in prostate cancer; therefore, CYTH-1 might serve as a new molecular target for the treatment of prostate cancer.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700005
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2011007500072
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.44 n.7 2011
Fonte: | info:eu-repo/semantics/openAccess