Data/hora: 10/06/2024 09:22:42

Provedor de dados: 	56	
País: 	Brazil	
Título: 	MicroRNA-4290 suppresses PDK1-mediated glycolysis to enhance the sensitivity of gastric cancer cell to cisplatin	
Autores: 	Qian,Yan; Wu,Xu; Wang,Haixiao; Hou,Guowei; Han,Xiao; Song,Wei.	
Data: 	2020-01-01	
Ano: 	2020	
Palavras-chave: 	MiR-4290; PDK1; Cisplatin; Chemoresistance; Gastric cancer.	
Resumo: 	The development of chemotherapy resistance significantly impairs the efficiency of chemotherapy, but the underlying mechanisms of chemotherapy resistance in gastric cancer (GC) are complicated and still need to be further explored. Here, we aimed to reveal the effects of miR-4290/PDK1 (pyruvate dehydrogenase kinase 1) axis on chemotherapy resistance of GC in vitro. The expression patterns of miR-4290 in GC tissues and cell lines were determined by real-time quantitative PCR. Kaplan-Meier was used to assess the relationship between miR-4290 expression levels and patients' overall survival. CCK-8 and flow cytometry technologies were applied to detect cell proliferation and apoptosis. The luciferase gene reporter assay was used to evaluate the interaction between miR-4290 and PDK1. miR-4290 was lowly expressed in GC tissues and cell lines, which was closely associated with the shorter overall survival of GC patients. miR-4290 mimics significantly inhibited cell proliferation and induced cell apoptosis, as well as induced a significant reduction in the expression of PDK1. Moreover, miR-4290 significantly inhibited glycolysis and decreased the IC50 value to cisplatin in SGC7901 cells, whereas these effects were abolished and cell apoptosis was promoted when PDK1 was overexpressed. In conclusion, this study revealed that miR-4290 suppressed PDK1-mediated glycolysis to enhance the sensitivity of GC cells to cisplatin.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500604	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/1414-431x20209330	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.53 n.5 2020	
Fonte: 	info:eu-repo/semantics/openAccess