Data/hora: 04/06/2024 00:49:55

Provedor de dados: | 56
País: | Brazil
Título: | Nitric oxide regulates angiotensin-I converting enzyme under static conditions but not under shear stress
Autores: | Pertrini,C.M.; Miyakawa,A.A.; Laurindo,F.R.M.; Krieger,J.E.
Data: | 2003-09-01
Ano: | 2003
Palavras-chave: | Shear stress; Angiotensin-I converting enzyme; Nitric oxide; Endothelial cells.
Resumo: | Mechanical forces including pressure and shear stress play an important role in vascular homeostasis via the control of the production and release of a variety of vasoactive factors. An increase in vascular shear stress is accompanied by nitric oxide (NO) release and NO synthase activation. Previously, we have demonstrated that shear stress induces angiotensin-I converting enzyme (ACE) down-regulation in vivo and in vitro. In the present study, we determined whether NO participates in the shear stress-induced ACE suppression response. Rabbit aortic endothelial cells were evaluated using the NO synthase inhibitor L-NAME, and two NO donors, diethylamine NONOate (DEA/NO) and sodium nitroprusside (SNP). Under static conditions, incubation of endothelial cells with 1 mM L-NAME for 18 h increased ACE activity by 27% (from 1.000 ± 0.090 to 1.272 ± 0.182) while DEA/NO and SNP (0.1, 0.5 and 1 mM) caused no change in ACE activity. Interestingly, ACE activity was down-regulated similarly in the presence or absence of L-NAME (delta(0 mM) = 0.26 ± 0.055, delta(0.1 mM) = 0.21 ± 0.22, delta(1 mM) = 0.36 ± 0.13) upon 18 h shear stress activation (from static to 15 dyn/cm²). Taken together, these results indicate that NO can participate in the maintenance of basal ACE levels in the static condition but NO is not associated with the shear stress-induced inactivation of ACE.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900005
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2003000900005
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.36 n.9 2003
Fonte: | info:eu-repo/semantics/openAccess