Data/hora: 12/06/2024 15:22:10

Provedor de dados: 	56	
País: 	Brazil	
Título: 	Study of a region on yeast chromosome XIII that complements pet G199 mutants (COX7) and carries a new non-essential gene	
Autores: 	Nobrega,M.P.; Graminha,M.A.S.; Troitskaya,E.N.; Nobrega,F.G.	
Data: 	1998-03-01	
Ano: 	1998	
Palavras-chave: 	Saccharomyces cerevisiae; Gene cloning; Cytochrome oxidase; Subunit VII; Gene disruption.	
Resumo: 	The mutants of Saccharomyces cerevisiae assigned to complementation group G199 are deficient in mitochondrial respiration and lack a functional cytochrome oxidase complex. Recombinant plasmids capable of restoring respiration were cloned by transformation of mutants of this group with a yeast genomic library. Sequencing indicated that a 2.1-kb subclone encompasses the very end (last 11 amino acids) of the PET111 gene, the COX7 gene and a new gene (YMR255W) of unknown function that potentially codes for a polypeptide of 188 amino acids (about 21.5 kDa) without significant homology to any known protein. We have shown that the respiratory defect corresponding to group G199 is complemented by plasmids carrying only the COX7 gene. The gene YMR255W was inactivated by one-step gene replacement and the disrupted strain was viable and unaffected in its ability to grow in a variety of different test media such as minimal or complete media using eight distinct carbon sources at three pH values and temperatures. Inactivation of this gene also did not affect mating or sporulation	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300004	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/S0100-879X1998000300004	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.31 n.3 1998	
Fonte: 	info:eu-repo/semantics/openAccess