Data/hora: 11/06/2024 10:12:49

Provedor de dados: | 56
País: | Brazil
Título: | Antifungal activity of the naphthoquinone beta-lapachone against disseminated infection with Cryptococcus neoformans var. neoformans in dexamethasone-immunosuppressed Swiss mice
Autores: | Medeiros,C.S.; Pontes-Filho,N.T.; Camara,C.A.; Lima-Filho,J.V.; Oliveira,P.C.; Lemos,S.A.; Leal,A.F.G.; Brandão,J.O.C.; Neves,R.P.
Data: | 2010-04-01
Ano: | 2010
Palavras-chave: | Beta-lapachone; Cryptococcus neoformans; Cryptococcosis; Immunesuppression; Experimental infection; Amphotericin B.
Resumo: | The in vivo antifungal activity of the naphthoquinone beta-lapachone against disseminated infection by Cryptococcus neoformans was investigated. Swiss mice were immunosuppressed daily with dexamethasone (0.5 mg per mouse) intraperitoneally for 3 days, the procedure was repeated 4 days later, and the animals were then challenged intravenously with C. neoformans (10(6) CFU/mL) 1 week later. Seven days after infection, the mice were divided into groups and treated daily with beta-lapachone (10 mg/kg, iv) for 7 (N = 6) and 14 days (N = 10). Amphotericin B (0.5 mg/kg) was used as comparator drug and an additional group received PBS. Treatment with beta-lapachone cleared the yeast from the spleen and liver, and the fungal burden decreased approximately 10(4) times in the lungs and brain 14 days after infection when compared to the PBS group (P &lt; 0.05). This result was similar to that of the amphotericin B-treated group. Protection was suggestively due to in vivo antifungal activity of this drug and apparently not influenced by activation of the immune response, due to similar leukocyte cell counts among all groups. This study highlights the prospective use of beta-lapachone for treatment of disseminated cryptococcosis.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400004
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2010007500012
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.43 n.4 2010
Fonte: | info:eu-repo/semantics/openAccess