Data/hora: 10/06/2024 10:00:31

Provedor de dados: | 56
País: | Brazil
Título: | Estrogen enhances vasoconstrictive remodeling after injury in male rabbits
Autores: | Francisco,Y.A.; Dantas,A.P.V.; Carvalho,M.H.C.; Laurindo,F.R.M.
Data: | 2005-09-01
Ano: | 2005
Palavras-chave: | Estrogen; Restenosis post-angioplasty; Nitric oxide; Endothelial function; Vascular remodeling.
Resumo: | The complete spectrum of estrogen vascular effects remains unclear. In particular, estrogen effects in the vascular response to profound injury in males have not been explored in detail. Therefore, we submitted 44 male New Zealand rabbits weighing 3.4 ± 0.6 kg to overdistention balloon injury of the right iliac artery. Rabbits were given 17ß-estradiol (5.45 µmol/day, sc) or vehicle for 7 days before and 14 days after injury, when the arteries were examined by post-mortem histomorphometry. Arteriographic caliber was assessed in vivo at baseline and before sacrifice. On day 14 after injury, in vivo arteriographic caliber (baseline = 2.44 ± 0.43 mm) was decreased by 23.1 ± 0.1% in controls and by 44.5 ± 0.1% in estrogen-treated rabbits (P &lt; 0.001). Neither the neointimal area nor the neointima/media area ratio changed after estrogen treatment. Collagen fraction was increased in the media and neointima of estrogen-treated rabbits vs control (1.38 ± 1.30 vs 0.35 ± 0.67, respectively, P = 0.01). Taken together, these findings suggest that estrogen increased negative vascular remodeling. Transcription of endothelial and inducible nitric oxide synthases (eNOS and iNOS) was analyzed by RT-PCR. eNOS mRNA expression was marginally increased after estrogen (P = 0.07) and injury. iNOS mRNA was increased 2- to 3-fold on day 14 after injury. With estrogen treatment, iNOS mRNA increased in uninjured arteries and exhibited a further 5.5-fold increase after injury. We concluded that estrogen increased lumen loss after balloon injury in male rabbits, likely by increased negative remodeling, which may be related to increased iNOS transcriptional rates.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900006
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2005000900006
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.38 n.9 2005
Fonte: | info:eu-repo/semantics/openAccess