Data/hora: 04/06/2024 15:39:58

Provedor de dados: | 56
País: | Brazil
Título: | Induced maturation of hepatic progenitor cellsin vitro
Autores: | Bi,Y.; He,Y.; Huang,J.Y.; Xu,L.; Tang,N.; He,T.C.; Feng,T.
Data: | 2013-07-01
Ano: | 2013
Palavras-chave: | Hepatic progenitor cells; Induction; Maturation; Dexamethasone; Hepatic growth factor; Fibroblast growth factor 4.
Resumo: | Hepatic progenitor cells (HPCs) are a potential cell source for liver cell transplantation but do not function like mature liver cells. We sought an effective and reliable method to induce HPC maturation. An immortalized HP14.5 albumin promoter-driven Gaussian luciferase (ALB-GLuc) cell line was established from HPCs isolated from fetal mouse liver of post coitus day 14.5 mice to investigate the effect of induction factors on ALB promoter. HP14.5 parental cells were cultured in DMEM with different combinations of 2% horse serum (HS), 0.1 µM dexamethasone (DEX), 10 ng/mL hepatic growth factor (HGF), and/or 20 ng/mL fibroblast growth factor 4 (FGF4). Trypan blue and crystal violet staining were used to assess cell proliferation with different induction conditions. Expression of hepatic markers was measured by semi-quantitative RT-PCR, Western blot, and immunofluorescence. Glycogen storage and metabolism were detected by periodic acid-Schiff and indocyanine green (ICG) staining. GLuc activity indicated ALB expression. The combination of 2% HS+0.1 µM Dex+10 ng/mL HGF+20 ng/mL FGF4 induced the highest ALB-GLuc activity. Cell proliferation decreased in 2% HS but increased by adding FGF4. Upon induction, and consistent with hepatocyte development, DLK, AFP, and CK19 expression decreased, while ALB, CK18, and UGT1A expression increased. The maturity markers tyrosine aminotransferase and apolipoprotein B were detected at days 3 and 6 post-induction, respectively. ICG uptake and glycogen synthesis were detectable at day 6 and increased over time. Therefore, we demonstrated that HPCs were induced to differentiate into functional mature hepatocytes in vitro, suggesting that factor-treated HPCs may be further explored as a means of liver cell transplantation.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000700559
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431X20132455
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.46 n.7 2013
Fonte: | info:eu-repo/semantics/openAccess