Data/hora: 16/06/2024 10:23:04

Provedor de dados: | 56
País: | Brazil
Título: | The biological effects of high-pressure gas on the yeast transcriptome
Autores: | Matsuoka,H.; Suzuki,Y.; Iwahashi,H.; Arao,T.; Suzuki,Y.; Tamura,K.
Data: | 2005-08-01
Ano: | 2005
Palavras-chave: | DNA microarray; Yeast; Hydrostatic pressure; Gas pressure; Nitrogen gas.
Resumo: | The aim of the present study was to examine the feasibility of DNA microarray technology in an attempt to construct an evaluation system for determining gas toxicity using high-pressure conditions, as it is well known that pressure increases the concentration of a gas. As a first step, we used yeast (Saccharomyces cerevisiae) as the indicator organism and analyzed the mRNA expression profiles after exposure of yeast cells to nitrogen gas. Nitrogen gas was selected as a negative control since this gas has low toxicity. Yeast DNA microarray analysis revealed induction of genes whose products were localized to the membranes, and of genes that are involved in or contribute to energy production. Furthermore, we found that nitrogen gas significantly affected the transport system in the cells. Interestingly, nitrogen gas also resulted in induction of cold-shock responsive genes. These results suggest the possibility of applying yeast DNA microarray to gas bioassays up to 40 MPa. We therefore think that "bioassays" are ideal for use in environmental control and protection studies.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800016
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2005000800016
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.38 n.8 2005
Fonte: | info:eu-repo/semantics/openAccess