Data/hora: 08/06/2024 09:19:05

Provedor de dados: | 56
País: | Brazil
Título: | The regulatory role of IL-6R in hepatitis B-associated fibrosis and cirrhosis
Autores: | Chen,Y.; Yang,S.; Peng,Y.; Yang,Z.
Data: | 2017-01-01
Ano: | 2017
Palavras-chave: | MiRNA-30b; IL-6 receptor; Chronic hepatitis B; Fibrosis; Cirrhosis.
Resumo: | This study investigated the expression and regulation of IL-6R in hepatitis B-associated moderate hepatic fibrosis and cirrhosis. Liver tissues, peripheral blood monocytes (PBMs) and serum were collected from 26 hepatitis B patients with liver fibrosis and 35 hepatitis B patients with liver cirrhosis. The levels of Il-6r mRNA expression in these samples were examined by quantitative real-time PCR and IL-6R protein levels were analyzed by western blot and ELISA. MiRNAs that regulate IL-6R expression were predicted by bioinformatics analysis, and validated by dual luciferase reporter assay. Compared with the hepatic fibrosis group, IL-6R was significantly upregulated at both mRNA and protein levels in liver tissues, PBMs and serum samples from the hepatic cirrhosis group (P&lt;0.05). The 3′UTR of Il-6r mRNA was predicted to contain a miR-30b binding site and IL-6R was identified as a possible target of miR-30b. MiR-30b expression was significantly downregulated in samples from hepatic cirrhosis patients compared with hepatic fibrosis patients (P&lt;0.05). In conclusion, IL-6R was upregulated while miR-30b was decreased in patients with liver cirrhosis. The miR-30 can directly regulate the expression of IL-6R.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001100610
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431x20176246
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.50 n.11 2017
Fonte: | info:eu-repo/semantics/openAccess