Data/hora: 09/06/2024 02:11:26

Provedor de dados: | 56
País: | Brazil
Título: | Modulation of staphylokinase-dependent plasminogen activation by mono- and divalent ions
Autores: | Yarzábal,A.; Serrano,R.L.; Puig,J.
Data: | 1999-01-01
Ano: | 1999
Palavras-chave: | Staphylokinase; Plasminogen activation; Fibrinolysis.
Resumo: | The effect of several ions (Cl-, Na+, K+, Ca2+) on the rate of plasminogen (Pg) activation by recombinant staphylokinase (rSTA) is reported. Both monovalent and divalent ions affect the rate at which Pg is activated by rSTA, in a concentration-dependent manner (range 0-100 mM). In almost all cases, a decrease of the initial velocity of activation was observed. Cl- showed the most striking inhibitory effect at low concentrations (64% at 10 mM). However, in the presence of a fibrin surface, this inhibition was attenuated to 38%. Surprisingly, 10 mM Ca2+ enhanced the Pg activation rate 21% when a polymerized fibrin matrix was present. These data support the idea that ions can modulate the rate of Pg activation through a mechanism that may be associated with changes in the molecular conformation of the zymogen. This effect is strongly dependent on the presence of a fibrin clot.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100005
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1999000100005
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.32 n.1 1999
Fonte: | info:eu-repo/semantics/openAccess