Data/hora: 10/06/2024 01:52:44

Provedor de dados: 	56	
País: 	Brazil	
Título: 	Modulation of staphylokinase-dependent plasminogen activation by mono- and divalent ions	
Autores: 	Yarzábal,A.; Serrano,R.L.; Puig,J.	
Data: 	1999-01-01	
Ano: 	1999	
Palavras-chave: 	Staphylokinase; Plasminogen activation; Fibrinolysis.	
Resumo: 	The effect of several ions (Cl-, Na+, K+, Ca2+) on the rate of plasminogen (Pg) activation by recombinant staphylokinase (rSTA) is reported. Both monovalent and divalent ions affect the rate at which Pg is activated by rSTA, in a concentration-dependent manner (range 0-100 mM). In almost all cases, a decrease of the initial velocity of activation was observed. Cl- showed the most striking inhibitory effect at low concentrations (64% at 10 mM). However, in the presence of a fibrin surface, this inhibition was attenuated to 38%. Surprisingly, 10 mM Ca2+ enhanced the Pg activation rate 21% when a polymerized fibrin matrix was present. These data support the idea that ions can modulate the rate of Pg activation through a mechanism that may be associated with changes in the molecular conformation of the zymogen. This effect is strongly dependent on the presence of a fibrin clot.	
Tipo: 	Info:eu-repo/semantics/other	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100005	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/S0100-879X1999000100005	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.32 n.1 1999	
Fonte: 	info:eu-repo/semantics/openAccess