Data/hora: 11/06/2024 11:51:45

Provedor de dados: | 56
País: | Brazil
Título: | Antisense mRNA for NPY-Y1 receptor in the medial preoptic area increases prolactin secretion
Autores: | Silveira,N.A.; Franci,C.R.
Data: | 1999-09-01
Ano: | 1999
Palavras-chave: | Neuropeptide Y; Medial preoptic area; Neuropeptide Y-Y1 receptor; Prolactin; MRNA for neuropeptide; Y-Y1 receptor; Luteinizing hormone; Follicle-stimulating hormone.
Resumo: | We investigated the participation of neuropeptide Y-Y1 receptors within the medial preoptic area in luteinizing hormone, follicle-stimulating hormone and prolactin release. Four bilateral microinjections of sense (control) or antisense 18-base oligonucleotides of messenger ribonucleic acid (mRNA) (250 ng) corresponding to the NH2-terminus of the neuropeptide Y1 receptor were performed at 12-h intervals for two days into the medial preoptic area of ovariectomized Wistar rats (N = 16), weighing 180 to 200 g, treated with estrogen (50 µg) and progesterone (25 mg) two days before the experiments between 8.00 and 10:00 a.m. Blockade of Y1 receptor synthesis in the medial preoptic area by the antisense mRNA did not change plasma luteinizing hormone or follicle-stimulating hormone but did increase prolactin from 19.6 ± 5.9 ng/ml in the sense group to 52.9 ± 9.6 ng/ml in the antisense group. The plasma hormones were measured by radioimmunoassay and the values are reported as mean ± SEM. These data suggest that endogenous neuropeptide Y in the medial preoptic area has an inhibitory action on prolactin secretion through Y1 receptors.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900016
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1999000900016
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.32 n.9 1999
Fonte: | info:eu-repo/semantics/openAccess