Data/hora: 08/06/2024 16:49:22

Provedor de dados: | 56
País: | Brazil
Título: | Propofol exerts anti-inflammatory effects in rats with lipopolysaccharide-induced acute lung injury by inhibition of CD14 and TLR4 expression
Autores: | Ma,Ling; Wu,Xiu-Ying; Zhang,Li-Hong; Chen,Wei-Min; Uchiyama,Akinori; Mashimo,Takashi; Fujino,Yuji.
Data: | 2013-03-01
Ano: | 2013
Palavras-chave: | CD14; Toll-like receptors; Propofol; Lipopolysaccharide; Lung injury.
Resumo: | We investigated the effect of propofol (Prop) administration (10 mg kg-1 h-1, intravenously) on lipopolysaccharide (LPS)-induced acute lung injury and its effect on cluster of differentiation (CD) 14 and Toll-like receptor (TLR) 4 expression in lung tissue of anesthetized, ventilated rats. Twenty-four male Wistar rats were randomly divided into three groups of 8 rats each: control, LPS, and LPS+Prop. Lung injury was assayed via blood gas analysis and lung histology, and tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) levels were determined in bronchoalveolar lavage fluid using ELISA. Real-time polymerase chain reaction was used to detect CD14 and TLR4 mRNA levels, and CD14 and TLR4 protein expression was determined by Western blot. The pathological scores were 1.2 ± 0.9, 3.3 ± 1.1, and 1.9 ± 1.0 for the control, LPS, and LPS+Prop groups, respectively, with statistically significant differences between control and LPS groups (P &lt; 0.05) and between LPS and LPS+Prop groups (P &lt; 0.05). The administration of LPS resulted in a significant increase in TNF-α and IL-1β levels, 7- and 3.5-fold, respectively (P &lt; 0.05), while treatment with propofol partially blunted the secretion of both cytokines (P &lt; 0.05). CD14 and TLR4 mRNA levels were increased in the LPS group (1.48 ± 0.05 and 1.26 ± 0.03, respectively) compared to the control group (1.00 ± 0.20 and 1.00 ± 0.02, respectively; P &lt; 0.05), while propofol treatment blunted this effect (1.16 ± 0.05 and 1.12 ± 0.05, respectively; P &lt; 0.05). Both CD14 and TLR4 protein levels were elevated in the LPS group compared to the control group (P &lt; 0.05), while propofol treatment partially decreased the expression of CD14 and TLR4 protein versus LPS alone (P &lt; 0.05). Our study indicates that propofol prevents lung injury, most likely by inhibition of CD14 and TLR4 expression.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300299
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431X20122379
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.46 n.3 2013
Fonte: | info:eu-repo/semantics/openAccess