Data/hora: 30/05/2024 11:34:27

Provedor de dados: | 56
País: | Brazil
Título: | Down-regulation of single-stranded DNA-binding protein 1 expression induced by HCMV infection promotes lipid accumulation in cells
Autores: | Guo,N.; Zhang,N.; Yan,L.; Cao,X.; Lv,F.; Wang,J.; Wang,Y.; Cong,H.
Data: | 2017-01-01
Ano: | 2017
Palavras-chave: | HCMV; SSBP1; HUVEC; Lipid accumulation; LDLR.
Resumo: | The objective of this study was to observe the infection of human cytomegalovirus (HCMV) to human umbilical vein endothelial cells, and its effect on the expression of single-stranded DNA-binding protein (SSBP1) and on lipid metabolism in endothelial cells. We screened the differential expression of mRNAs after HCMV infection by suppression subtractive hybridization and the expression levels of SSBP1 mRNA and protein after HCMV infection by real-time PCR and western blot. After verification of successful infection by indirect immunofluorescent staining and RT-PCR, we found a differential expression of lipid metabolism-related genes including LDLR, SCARB, CETP, HMGCR, ApoB and LPL induced by HCMV infection. The expression levels of SSBP1 mRNA and protein after HCMV infection were significantly down-regulated. Furthermore, we found that upregulation of SSBP1 inhibited the expression of atherosclerosis-associated LDLR, SCARB, HMGCR, CETP as well as the accumulation of lipids in the cells. The results showed that the inhibition of SSBP1 by HCMV infection promotes lipid accumulation in the cells.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001100605
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431x20176389
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.50 n.11 2017
Fonte: | info:eu-repo/semantics/openAccess