Data/hora: 12/06/2024 02:22:58

Provedor de dados: | Brazilian Journal of Medical and Biological Research
País: | Brazil
Título: | Effect of a submaxillary gland extract on Ehrlich tumor growth in mice
Autores: | Weill,P.; Frussa-Filho,R.; Bonamin,L.V.
Data: | 1999-10-01
Ano: | 1999
Palavras-chave: | Ehrlich ascites carcinoma; Submaxillary salivary gland; Growth factors.
Resumo: | Ablation of host submaxillary glands modifies Ehrlich tumor growth and tumor-infiltrating leukocytes, possibly by modifications in the serum level of growth factors produced by this gland. To extend this research, 7-month-old male EPM-1 mice (N = 30) were divided into two groups: 1) inoculated with tumor cells previously incubated with submaxillary salivary gland extract (SGE) in PBS for 30 min at 37%; 2) inoculated with tumor cells previously incubated with PBS, under the same conditions. Animals were inoculated into the footpad with 40 µl of a suspension containing 4.5 x 107 tumor cells/ml, and footpad thickness was measured daily for 10 days. Sections and smears of tumor cells were prepared from the tumor mass to determine mitosis frequency, percent of tumor cells immunopositive to nerve (NGF) and epidermal (EGF) growth factors and percent of tumor-infiltrating leukocytes. The incubation of tumor cells with SGE produced a tumor reduction of about 30% in size (P&amp;lt;0.01). This effect was not related to loss of cell viability during incubation, but a 33% increase (P&amp;lt;0.05) in the percentage of dead or dying tumor cells and a 15% increase in the percent of NGF/EGF-positive tumor cells (P&amp;lt;0.01) were observed in vivo at the end of experiment. Tumor-infiltrating lymphocytes and mitosis frequency did not differ between groups. These data suggest a direct effect of factors present in SGE on tumor cells, which induce degeneration of tumor cells.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000005
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1999001000005
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.32 n.10 1999
Fonte: | info:eu-repo/semantics/openAccess