Data/hora: 10/06/2024 12:20:22

Provedor de dados: 	Brazilian Journal of Medical and Biological Research	
País: 	Brazil	
Título: 	Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons	
Autores: 	Mafra,R.A.; Leão,R.M.; Beirão,P.S.L.; Cruz,J.S.	
Data: 	2003-07-01	
Ano: 	2003	
Palavras-chave: 	Cerebellar granule neurons; Excitatory amino-acid transporter type 2; Patch-clamp; Glutamate; Whole-cell voltage-clamp.	
Resumo: 	A glutamate-sensitive inward current (Iglu) is described in rat cerebellar granule neurons and related to a glutamate transport mechanism. We examined the features of Iglu using the patch-clamp technique. In steady-state conditions the Iglu measured 8.14 ± 1.9 pA. Iglu was identified as a voltage-dependent inward current showing a strong rectification at positive potentials. L-Glutamate activated the inward current in a dose-dependent manner, with a half-maximal effect at about 18 µM and a maximum increase of 51.2 ± 4.4%. The inward current was blocked by the presence of dihydrokainate (0.5 mM), shown by others to readily block the GLT1 isoform. We thus speculate that Iglu could be attributed to the presence of a native glutamate transporter in cerebellar granule neurons.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700018	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/S0100-879X2003000700018	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.36 n.7 2003	
Fonte: 	info:eu-repo/semantics/openAccess