Data/hora: 12/06/2024 14:15:46

Provedor de dados: | Brazilian Journal of Microbiology
País: | Brazil
Título: | Comparison of three decontamination methods for Mycobacterium bovis isolation
Autores: | Ambrosio,Simone Rodrigues; Oliveira,Eugenia Márcia de Deus; Rodriguez,Cesar Alejandro Rosales; Ferreira Neto,José Soares; Amaku,Marcos.
Data: | 2008-06-01
Ano: | 2008
Palavras-chave: | Mycobacterium bovis; Decontamination; Petroff; HPC.
Resumo: | Sixty samples of tissue fragments with lesions suggestive of tuberculosis from bovine abattoirs, kept in saturated solution of sodium borate, were subjected to four treatments: 4% NaOH (Petroff Method), 12 % H2SO4 and 1.5% HPC (1-Hexadecylpyridinium Chloride) decontamination, and physiological saline solution (control). The HPC method showed the lowest contamination rate (3%) when compared to control (88%, p&lt;0.001), NaOH (33%, p&lt;0.001) and H2SO4 (21.7%, p&lt;0.002). Regarding the isolation success, the HPC method was better (40%) than the control (3%, p&lt;0.001), NaOH (13%, p=0.001) and H2SO4 (1.7%, p&lt;0.001) methods. These results indicate that HPC is an alternative to the Petroff method.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200008
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822008000200008
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.39 n.2 2008
Fonte: | info:eu-repo/semantics/openAccess