Data/hora: 09/06/2024 13:29:21

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Comparison of three decontamination methods for Mycobacterium bovis isolation	
Autores: 	Ambrosio,Simone Rodrigues; Oliveira,Eugenia Márcia de Deus; Rodriguez,Cesar Alejandro Rosales; Ferreira Neto,José Soares; Amaku,Marcos.	
Data: 	2008-06-01	
Ano: 	2008	
Palavras-chave: 	Mycobacterium bovis; Decontamination; Petroff; HPC.	
Resumo: 	Sixty samples of tissue fragments with lesions suggestive of tuberculosis from bovine abattoirs, kept in saturated solution of sodium borate, were subjected to four treatments: 4% NaOH (Petroff Method), 12 % H2SO4 and 1.5% HPC (1-Hexadecylpyridinium Chloride) decontamination, and physiological saline solution (control). The HPC method showed the lowest contamination rate (3%) when compared to control (88%, p&lt;0.001), NaOH (33%, p&lt;0.001) and H2SO4 (21.7%, p&lt;0.002). Regarding the isolation success, the HPC method was better (40%) than the control (3%, p&lt;0.001), NaOH (13%, p=0.001) and H2SO4 (1.7%, p&lt;0.001) methods. These results indicate that HPC is an alternative to the Petroff method.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200008	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822008000200008	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.39 n.2 2008	
Fonte: 	info:eu-repo/semantics/openAccess