Data/hora: 09/06/2024 15:45:05

Provedor de dados: | 58
País: | Brazil
Título: | Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samples
Autores: | Cruz,Heidi Lacerda Alves da; Montenegro,Rosana de Albuquerque; Lima,Juliana Falcão de Araújo; Poroca,Diogo da Rocha; Lima,Juliana Figueirêdo da Costa; Montenegro,Lílian Maria Lapa; Crovella,Sergio; Schindler,Haiana Charifker.
Data: | 2011-03-01
Ano: | 2011
Palavras-chave: | Mycobacterium tuberculosis; Nested-PCR; Blood; Urine; Molecular diagnosis; IS6110 insertion sequence.
Resumo: | The polymerase chain reaction (PCR) and its variations, such as the nested-PCR, have been described as promising techniques for rapid diagnosis of tuberculosis (TB). With the aim of evaluating the usefulness of a nested-PCR method on samples of blood and urine of patients suspected of tuberculosis we analyzed 192 clinical samples, using as a molecular target the insertion element IS6110 specific of M. tuberculosis genome. Nested-PCR method showed higher sensitivity in patients with extrapulmonary tuberculosis (47.8% and 52% in blood and urine) when compared to patients with the pulmonary form of the disease (sensitivity of 29% and 26.9% in blood and urine), regardless of the type of biological sample used. The nested-PCR is a rapid technique that, even if not showing a good sensitivity, should be considered as a helpful tool especially in the extrapulmonary cases or in cases where confirmatory diagnosis is quite difficult to be achieved by routine methods. The performance of PCR-based techniques should be considered and tested in future works on other types of biological specimens besides sputum, like blood and urine, readily obtainable in most cases. The improving of M. tuberculosis nested-PCR detection in TB affected patients will give the possibility of an earlier detection of bacilli thus interrupting the transmission chain of the disease.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100041
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822011000100041
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.42 n.1 2011
Fonte: | info:eu-repo/semantics/openAccess