Data/hora: 09/06/2024 04:34:06

Provedor de dados: | Brazilian Journal of Microbiology
País: | Brazil
Título: | In vitro biofilm forming potential of Streptococcus suis isolated from human and swine in China
Autores: | Dawei,Guo; Liping,Wang; Chengping,Lu.
Data: | 2012-09-01
Ano: | 2012
Palavras-chave: | Biofilm; Streptococcus suis; Scanning electron microscopy; Confocal laser scanning microscopy; Microplate assay; Antimicrobial agents.
Resumo: | Streptococcus suis is a swine pathogen and also a zoonotic agent. The formation of bioﬁlms allows S. suis to become persistent colonizers and resist clearance by the host immune system and antibiotics. In this study, biofilm forming potentials of various S. suis strains were characterized by confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM) and tissue culture plates stained with crystal violet. In addition, the effects of five antimicrobial agents on biofilm formation were assayed in this study. S. suis produced biofilms on smooth and rough surface. The nutritional contents including glucose and NaCl in the growth medium modulated biofilm formation. There was a significant difference in their biofilm-forming ability among all 46 S. suis strains. The biofilm-forming potential of S. suis serotype 9 was stronger than type 2 and all other types. However, biofilm formation was inhibited by five commonly used antimicrobial agents, penicillin, erythromycin, azithromycin, ciprofloxacin, and ofloxacin at subinhibitory concentrations, among which inhibition of ciprofloxacin and ofloxacin was stronger than that of other three antimicrobial agents.Our study provides a detailed analysis of biofilm formation potential in S. suis, which is a step towards understanding its role in pathogenesis, and eventually lead to a better understanding of how to eradicate S. suis growing as biofilms with antibiotic therapy.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300021
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822012000300021
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.43 n.3 2012
Fonte: | info:eu-repo/semantics/openAccess