Data/hora: 13/06/2024 05:18:19

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Genetic analysis of Escherichia coli strains carrying enteropathogenic Escherichia coli (EPEC) markers, isolated from children in Rio de Janeiro city, Brazil	
Autores: 	Regua-Mangia,Adriana Hamond; Gomes,Tânia Aparecida Tardelli; Andrade,João Ramos Costa; Vieira,Mônica Aparecida Midolli; Gonzalez,Alice Gonçalves Martins; Zahner,Viviane; Irino,Kinue; Teixeira,Lúcia Martins.	
Data: 	2003-11-01	
Ano: 	2003	
Palavras-chave: 	Enteropathogenic Escherichia coli; Genetic diversity; Virulence markers.	
Resumo: 	In the present study, 47 enteropathogenic Escherichia coli strains identified according to serotyping, presence of eae, bfp and EAF sequences, adherence phenotype and ability to induce attaching-effacing lesions were analyzed by pulsed-field gel electrophoresis (PFGE), multilocus enzyme electrophoresis (MLEE), and the presence of LEE genes (eae, espA, espB, tir) as well as the respective alleles. Amplification of LEE genes subtypes revealed 18 different pathotypes. Typing of the eae gene showed that most strains contained nontypable intimin (42%) followed by beta (35%), gamma and alpha genes (12% each). PFGE analysis revealed a variable degree of polymorphism among isolates and, in general, no clear correlation was observed among PFGE profiles and the virulence markers identified. Otherwise, grouping based on MLEE analysis showed a close association between eae allele and clonal cluster distribution leading us to indicate the eae profile as a promising marker to establish relatedness among such microorganisms.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500013	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822003000500013	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.34 suppl.1 2003	
Fonte: 	info:eu-repo/semantics/openAccess