Data/hora: 04/06/2024 01:31:58

Provedor de dados: | Brazilian Journal of Microbiology
País: | Brazil
Título: | Validation of a real-time PCR assay for the molecular identification of Mycobacterium tuberculosis
Autores: | Sales,Mariana L.; Fonseca Júnior,Antônio Augusto; Orzil,Lívia; Alencar,Andrea Padilha; Silva,Marcio Roberto; Issa,Marina Azevedo; Soares Filho,Paulo Martins; Lage,Andrey Pereira; Heinemann,Marcos Bryan.
Data: | 2014-12-01
Ano: | 2014
Palavras-chave: | Mycobacterium tuberculosis; Diagnosis; Real time PCR.
Resumo: | Mycobacterium tuberculosis is the major cause of tuberculosis in humans. This bacillus gained prominence with the occurrence of HIV, presenting itself as an important opportunistic infection associated with acquired immunodeficiency syndrome (AIDS). The current study aimed to develop a real-time PCR using Eva Green technology for molecular identification of M. tuberculosis isolates. The primers were designed to Rv1510 gene. Ninety nine samples of M. tuberculosis and sixty samples of M. bovis were tested and no sample of the bovine bacillus was detected by the qPCR. Statistical tests showed no difference between the qPCR and biochemical tests used to identify the Mycobacterium tuberculosis. The correlation between tests was perfect with Kappa index of 1.0 (p &lt; 0.001, CI = 0.84 - 1.0). The diagnostic sensitivity and specificity were 100% (CI = 95.94% - 100%) and 100% (CI = 93.98% - 100%). This qPCR was developed with the goal of diagnosing the bacillus M. tuberculosis in samples of bacterial suspension. TB reference laboratories (health and agriculture sectors), public health programs and epidemiological studies probably may benefit from such method.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400029
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822014000400029
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.45 n.4 2014
Fonte: | info:eu-repo/semantics/openAccess