Data/hora: 15/06/2024 22:39:31

Provedor de dados: | 58
País: | Brazil
Título: | Applications of image analysis in the characterization of Streptomyces olindensis in submerged culture
Autores: | Pamboukian,Celso R. Denser; Guimarães,Luciana M.; Facciotti,Maria Cândida R.
Data: | 2002-01-01
Ano: | 2002
Palavras-chave: | Streptomyces olindensis; Image analysis; Morphology.
Resumo: | The morphology of Streptomyces olindensis (producer of retamycin, an antitumor antibiotic) grown in submerged culture was assessed by image analysis. The morphology was differentiated into four classes: pellets, clumps (or entangled filaments), branched and unbranched free filaments. Four morphological parameters were initially considered (area, convex area, perimeter, and convex perimeter) but only two parameters (perimeter and convex perimeter) were chosen to automatically classify the cells into the four morphological classes, using histogram analysis. Each morphological class was evaluated during growth carried out in liquid media in fermenter or shaker. It was found that pellets and clumps dominated in early growth stages in fermenter (due to the inoculum coming from a shaker cultivation) and that during cultivation, the breakage of pellets and clumps caused an increase in the percentage of free filaments. The criteria of morphological classification by image analysis proposed were useful to quantify the percentage of each morphological class during fermentations and may help to establish correlations between antibiotic production and microorganism morphology.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100003
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822002000100003
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.33 n.1 2002
Fonte: | info:eu-repo/semantics/openAccess