Data/hora: 08/06/2024 15:36:01

Provedor de dados: | 58
País: | Brazil
Título: | Genetic profiling of Klebsiella pneumoniae: comparison of pulsed field gel electrophoresis and random amplified polymorphic DNA
Autores: | Ashayeri-Panah,Mitra; Eftekhar,Fereshteh; Ghamsari,Maryam Mobarak; Parvin,Mahmood; Feizabadi,Mohammad Mehdi.
Data: | 2013-09-01
Ano: | 2013
Palavras-chave: | Klebsiella pneumoniae; Pulsed field gel electrophoresis; Random amplified polymorphic DNA; Genotyping.
Resumo: | In this study, the discriminatory power of pulsed field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) methods for subtyping of 54 clinical isolates of Klebsiella pneumoniae were compared. All isolates were typeable by RAPD, while 3.6% of them were not typeable by PFGE. The repeatability of both typing methods were 100% with satisfying reproducibility (≥ 95%). Although the discriminatory power of PFGE was greater than RAPD, both methods showed sufficient discriminatory power (DI &gt; 0.95) which reflects the heterogeneity among the K. pneumoniae isolates. An optimized RAPD protocol is less technically demanding and time consuming that makes it a reliable typing method and competitive with PFGE.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300024
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822013005000055
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.44 n.3 2013
Fonte: | info:eu-repo/semantics/openAccess