Data/hora: 16/06/2024 10:45:56

Provedor de dados: | 58
País: | Brazil
Título: | Endo-and exoglucanase activities in bacteria from mangrove sediment
Autores: | Soares Júnior,Fábio Lino; Dias,Armando Cavalcante Franco; Fasanella,Cristiane Cipola; Taketani,Rodrigo Gouvêa; Lima,André Oliveira de Souza; Melo,Itamar Soares; Andreote,Fernando Dini.
Data: | 2013-09-01
Ano: | 2013
Palavras-chave: | Cellulose endo-1; 4-β-D-glucanase exo-1; 4-β-D-glucanase salinity.
Resumo: | The mangrove ecosystem is an unexplored source for biotechnological applications. In this unique environment, endemic bacteria have the ability to thrive in the harsh environmental conditions (salinity and anaerobiosis), and act in the degradation of organic matter, promoting nutrient cycles. Thus, this study aimed to assess the cellulolytic activities of bacterial groups present in the sediment from a mangrove located in Ilha do Cardoso (SP, Brazil). To optimize the isolation of cellulolytic bacteria, enrichments in two types of culture media (tryptone broth and minimum salt medium), both supplemented with 5% NaCl and 1% of cellulose, were performed. Tests conducted with the obtained colonies showed a higher occurrence of endoglycolytic activity (33 isolates) than exoglycolytic (19 isolates), and the degradation activity was shown to be modulated by the presence of NaCl. The isolated bacteria were clustered by BOX-PCR and further classified on the basis of partial 16S rRNA sequences as Alphaproteobacteria, Gammaproteobacteria, Actinobacteria, Firmicutes or Bacteroidetes. Therefore, this study highlights the importance of studies focusing on the endemic species found in mangroves to exploit them as novel biotechnological tools for the degradation of cellulose.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300048
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822013000300048
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.44 n.3 2013
Fonte: | info:eu-repo/semantics/openAccess