Data/hora: 06/06/2024 03:46:51

Provedor de dados: | Brazilian Journal of Microbiology
País: | Brazil
Título: | Production of inulinase from Kluyveromyces marxianus using Dahlia tuber extract
Autores: | Jain,Sumat Chand; Jain,P.C.; Kango,Naveen.
Data: | 2012-03-01
Ano: | 2012
Palavras-chave: | Inulinase; Dahlia; Fructose; Kluyveromyces marxianus.
Resumo: | Various carbon sources were evaluated for production of inulinase by yeast, Kluyveromyces marxianus MTCC 3995. Highest inulinase activity was observed with Dahlia extract (25.3 nkat mL-1) as carbon source. The enzyme activity was 1.4 folds higher than that observed in media containing pure chicory inulin (17.8 nkat mL-1). The yeast showed good growth on a simple medium containing dahlia extract (20% w/v) and yeast extract (2%w/v) as carbon and nitrogen source respectively, in 96 h. at 28°C and 120 rpm. Lowest inulinase yield (4.8 nkat mL-1) was seen in the medium containing glucose as C-source. Although varied inulinase levels were noticed on different C- sources, Inulinase: Sucrase (I/S) ratios were noticed to be similar. Among various protein sources tested, yeast extract was found to be the best source followed by beef extract (17.9 nkat mL-1) and peptone (13.8 nkat mL-1). The enzyme was optimally active at pH (4.0) and 50°C. TLC analysis of end product revealed that inulinase hydrolyzed inulin exclusively into fructose. Results suggest that the dahlia extract induced exoinulinase synthesis in Kluyveromyces marxianus and can be utilized as a potential substrate for inulinase production.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100007
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822012000100007
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.43 n.1 2012
Fonte: | info:eu-repo/semantics/openAccess