Data/hora: 17/06/2024 03:41:42

Provedor de dados: 	58	
País: 	Brazil	
Título: 	A plate method for rapid screening of Ketogulonicigenium vulgare mutants for enhanced 2-keto-l-gulonic acid production	
Autores: 	Yang,Weichao; Han,Litao; Mandlaa,Mandlaa; Zhang,Haihong; Zhang,Zhongze; Xu,Hui.	
Data: 	2017-07-01	
Ano: 	2017	
Palavras-chave: 	Ketogulonicigenium vulgare; PH indicator; Rapid screening; Spaceflight mutation; 2-Keto-l-gulonic acid.	
Resumo: 	Abstract A new plate method was developed for rapid screening of Ketogulonicigenium vulgare mutants overproducing 2-keto-l-gulonic acid (2-KLG). The screening methodology took the advantage of the acidity caused by 2-KLG, which changes the color of bromothymol blue (pH indicator) from blue to yellow. Using the proposed method, a mutant, K. vulgare 65, was selected from 20,000 colonies produced by a strain subjected to spaceflight mutagenesis. When co-cultured with Bacillus megaterium 2980 in 20-L fermenters, K. vulgare 65 showed a high conversion rate (94.45%) of l-sorbose to 2-KLG. In contrast to the traditional screening method, this one significantly improved the frequency of obtaining positive mutants. The proposed plate screening method is cost-effective and easy to run and is thus useful for the isolation and screening of K. vulgare mutants overproducing 2-KLG.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300397	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1016/j.bjm.2017.02.002	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.48 n.3 2017	
Fonte: 	info:eu-repo/semantics/openAccess