Data/hora: 05/06/2024 11:37:31

Provedor de dados: | Brazilian Journal of Microbiology
País: | Brazil
Título: | Development of a transformation system for Penicillium brevicompactum based on the Fusarium oxysporum nitrate reductase gene
Autores: | Varavallo,Maurílio Antônio; Queiroz,Marisa Vieira de; Pereira,Jorge Fernando; Ribeiro,Ronney Adriano; Soares,Marcos Antônio; Ribeiro,João Batista; Araújo,Elza Fernandes de.
Data: | 2005-06-01
Ano: | 2005
Palavras-chave: | Nitrate reductase; Penicillium brevicompactum; Heterologous transformation.
Resumo: | Penicillium brevicompactum is a filamentous fungus that presents a potential for industrial use due its efficient pectinase production. A heterologous transformation system was developed for P. brevicompactum based on the complementation of a nitrate reductase mutant. Nitrate reductase mutants were obtained by resistance to chlorate in a rate of 23.24% when compared to other mutations that lead to the chlorate resistance. One mutant named 4457-18X was chosen for the transformation experiments with the pNH24 vector containing de Fusarium oxysporum nitrate reductase gene. A frequency of approximately 3 transformants/µg DNA was obtained using the circular vector pNH24. This frequency was multiplied about 10 fold using the linearized vector with the Xba I restriction enzyme. Southern analysis of the transformants showed a tendency of the linearized vector to diminish the number of integrations compared to the use of the circular vector. The integration was random and stable in the analyzed transformants. The establishment of a transformation system for P. brevicompactum is fundamental for genetic manipulation of this microorganism.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200015
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822005000200015
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.36 n.2 2005
Fonte: | info:eu-repo/semantics/openAccess